Local irritation test for DNA recombinant Porcine Somatotrophin(PST) were performed in the New Zealand White rabbits.
According to the Established Regulations of National Institute of Safety Research, the eye irritation test of PST was performed for 7 days in 9 New Zealand White Rabbits. PST (80㎎/vial)was diluted with 10㎖ saline and 0.1㎖ PST was dropped on the right cornea in each rabbit. After 20-30 seconds, three rabbits´ eyes were cleaned with warm saline for I minute. Other 6 rabbits´ eyes were left uncleaned. The untreated left eyes were negative control. The lesions of cornea, iris and conjuntiva were assessed by the Grade Table from the Regulation of National Institute of Safety Research. It was concluded that PST had no eye irritation.
The skin irritation test of PST was performed for 7 days in New Zealand White rabbits based on the Established Regulation of National Institute of Safety Research. During observation period, no significant clinical symptoms were observed. Significant changes such as erythema, scar tissue and edema were not shown on the sites applied.
The subcutaneous and muscular irritation test of PST was performed in nine New Zealand White rabbits. PST of ten times of the clinical dose was administreated subcutaneously in the dosal area and musculary in the gluteal muscle. Can the 7th day after appling PST, 6 rabbits were euthanized for histopathological examination. In histologieal examination, subcutaneous and muscular tissues were edematous and infiltrated by inflammatory cells respectively. On 7th day after appling PST, the other rabbits were euthanized for histopathological examination. In the histopatholagical examination, slight lymphocytes infiltration of the subcutaneous tissues and normal muscular tissues were observed.
From the above results, it was concluded that PST has no irritation on local areas such as eye, skin and subcutaneous or muscular tissues.
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